Channelpedia

CHO

Description: Chinese Hamster Ovary Cells

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Introduction

CHO cells are a line of epithelial-like cells that was initiated in 1957 by T.T. Puck and colleagues at the Department of Biophysics, University of Colorado Medical School in Denver, USA from a biopsy of an ovary of an adult Chinese hamster [1352]. There are several specific features that make the CHOcell line one of the most convenient solutions for reconstitution of mammalian ionchannels and related proteins: 1) it is a mammalian cell line; 2) they are adherent cells that make them easier to transfect compared to cell lines that grow in suspension; 3) cells are not electrotonically coupled and so are easy to voltage clamp, and 4) they express very little endogenous currents. [1353]


Experimental data


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Morphology


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Genes


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Proteins


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Ion channels

TRPM4, KCNK5, Kir, Neglegible Ik.


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Function


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Electrophysiology


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Model


References

1353

1354

Krafte DS. et al. Characterization of endogenous sodium channel gene expressed in Chinese hamster ovary cells.
Am. J. Physiol., 1993 Apr , 264 (C803-9).

1355

Hong SG. et al. Endogenous TRPM4-like channel in Chinese hamster ovary (CHO) cells.
Biochem. Biophys. Res. Commun., 2008 May 2 , 369 (712-7).


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