Channelpedia

Slo3

Description: potassium large conductance pH-sensitive channel, subfamily M, alpha member 3
Gene: Kcnma3     Synonyms: Slo3, kcnu1, Slo3, Kcnma3, KCa5

Edit - History

Introduction

The mouse Slo3 gene (KCNMA3) encodes a K+ channel that is regulated by changes in cytosolic pH. Like Slo1 subunits responsible for the Ca2+ and voltage-activated BK-type channel, the Slo3 α subunit contains a pore module with homology to voltage-gated K+ channels and also an extensive cytosolic C terminus thought to be responsible for ligand dependence. For the Slo3 K+ channel, increases in cytosolic pH promote channel activation, but very little is known about many fundamental properties of Slo3 currents. (Zhang [165])


Experimental data


Edit - History

Gene

Slo3 shares the most extensive sequence homology with Slo1 (Schreiber 1998 [1160]).

RGD ID Chromosome Position Species
1305519 - Rat

Kcnma3 : potassium large conductance pH-sensitive channel, subfamily M, alpha member 3


Edit

Transcript


Edit

Ontology


Edit - History

Interaction

In contrast to Slo1, Slo3 is insensitive to cytosolic Ca2+ and appears sensitive to cytosolic pH (Schreiber et al., 1998 [1160]; Xia et al., 2004 [164]).


Edit

Protein


Edit - History

Structure

K+ channels formed from α subunits encoded by the Slo gene family (Adelman et al., 1992 [1156]; Butler et al., 1993 [1157]) are characterized by a large cytosolic C-terminal structure that is thought to be involved in ligand-dependent regulation of the channels (Wei et al., 1994 [1158]; Schreiber and Salkoff [1159], 1997; Schreiber et al. , 1998 [1160]; Bhattacharjee et al., 2002 [1161]; Jiang et al., 2002 [625]; Shi et al., 2002 [1131]; Xia et al., 2002 [1136]; Bhattacharjee et al., 2003 [1141]; Yuan et al., 2003 [1139]; Xia et al., 2004 [164]). In fact, the hallmark for each member of the Slo family appears to be regulation by a specific cytosolic ligand: pH for Slo3 (Schreiber et al., 1998 [1160]; Xia et al., 2004 [164]).


Edit

Distribution


Edit - History

Expression

In contrast to the wide distribution of channels arising from either Slo1 and Slo2.x subunits, Slo3 has a much more limited pattern of expression than the other family members, being found predominantly in mouse testis (Schreiber et al., 1998 [1160]).


Edit

Functional


Edit - History

Kinetics

Slo1 and Slo3 differ in two primary ways: first, the intrinsic voltage dependence of the Slo3 closed–open equilibrium is weaker, and, second, voltage sensor movement in Slo3 is much more weakly coupled to channel opening. Both activation and deactivation are best described by two exponential components, both of which are only weakly voltage dependent. Qualitatively, the properties of the two kinetic components in the activation time course suggest that increases in pH increase the fraction of more rapidly opening channels. (Zhang [165])


Edit

Model


References

165

Zhang X. et al. Slo3 K+ channels: voltage and pH dependence of macroscopic currents.
J. Gen. Physiol., 2006 Sep , 128 (317-36).

1156

1157

Salkoff L. et al. mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels.
Science, 1993 Jul 9 , 261 (221-4).

1158

Salkoff L. et al. Calcium sensitivity of BK-type KCa channels determined by a separable domain.
Neuron, 1994 Sep , 13 (671-81).

1159

Schreiber M. et al. Transplantable sites confer calcium sensitivity to BK channels.
Nat. Neurosci., 1999 May , 2 (416-21).

1160

Schreiber M. et al. Slo3, a novel pH-sensitive K+ channel from mammalian spermatocytes.
J. Biol. Chem., 1998 Feb 6 , 273 (3509-16).

1161

Kaczmarek LK. et al. Localization of the Slack potassium channel in the rat central nervous system.
J. Comp. Neurol., 2002 Dec 16 , 454 (241-54).

625

MacKinnon R. et al. Crystal structure and mechanism of a calcium-gated potassium channel.
Nature, 2002 May 30 , 417 (515-22).

1132

Shi J. et al. Mechanism of magnesium activation of calcium-activated potassium channels.
Nature, 2002 Aug 22 , 418 (876-80).

1136

1141

Kaczmarek LK. et al. Slick (Slo2.1), a rapidly-gating sodium-activated potassium channel inhibited by ATP.
J. Neurosci., 2003 Dec 17 , 23 (11681-91).

1139


Edit

Credits