Channelpedia

BKB3

Description: potassium large conductance calcium-activated channel, subfamily M beta member 3
Gene: Kcnmb3     Synonyms: kcnmb3

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Introduction

Until 1999, only one beta subunit of BK (MaxiK) channels was known at the molecular level. This subunit (now known as the Beta-1-subunit) accounted mainly for MaxiK properties in vascular smooth muscle cells (VSMCs), where it is highly expressed. In the following years, three more beta subunits have been cloned and characterized. (Orio [540])

KCNMB3 (also known as HBETA3; KCNMB2; KCNMBL; BKBETA3; SLOBETA3) encodes BKB3, potassium large conductance calcium-activated channel (subfamily M) beta member 3. MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. BKB3 may partially inactivate or slightly decrease the activation time of MaxiK alpha subunit currents. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 22.

http://www.ncbi.nlm.nih.gov/gene/27094


Experimental data


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Gene

The b3-subunit (BKB3) was cloned from human EST databases and is phylogenetically more related to b2 than to b1 (Uebele [1179]). There are four splice variants (BKB3a-d), whose differences are in the NH2-terminal region. (Orio [540])

The mouse beta3 subunit, excluding N-terminal splice variants, shares only 62.8% amino acid identity with its human counterpart. (Zheng [1184])

RGD ID Chromosome Position Species
1311852 2 118679363-118693857 Rat
1621871 3 32371243-32390891 Mouse
1323526 3 178957537-178984838 Human

Kcnmb3 : potassium large conductance calcium-activated channel, subfamily M beta member 3


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Transcript

Acc No Sequence Length Source
NM_001104560 n/A n/A NCBI
NM_001195074 n/A n/A NCBI
NM_171830 n/A n/A NCBI
NM_014407 n/A n/A NCBI
NM_171828 n/A n/A NCBI
NM_001163677 n/A n/A NCBI
NM_171829 n/A n/A NCBI

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Ontology

Accession Name Definition Evidence
GO:0016021 integral to membrane Penetrating at least one phospholipid bilayer of a membrane. May also refer to the state of being buried in the bilayer with no exposure outside the bilayer. When used to describe a protein, indicates that all or part of the peptide sequence is embedded in the membrane. IEA
GO:0016020 membrane Double layer of lipid molecules that encloses all cells, and, in eukaryotes, many organelles; may be a single or double lipid bilayer; also includes associated proteins. IEA

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Interaction


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Protein


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Structure

The topology of alpha and beta subunits that make up BK channels can be seen in fig. 1 of Orio [540]. The channel is formed by 4 alpha-subunits and probably 4 beta subunits. Regulatory beta subunits share a putative membrane topology, with two transmembrane segments connected by a 120- residue extracellular “loop” and with NH2 and COOH terminals oriented toward the cytoplasm (Fig. 1 [540]). The loop has three or four putative glycosylation sites. Four beta subunits have been cloned in mammals (Brenner [1181], Knaus [1167], Uebele [1179], Xia [1182]).

BKB3 is phylogenetically more related to BKB2 than to BKB1 (Uebele [1179]). There are four splice variants (BKB3a-d), whose differences are in the NH2-terminal region. (Orio [540])


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Distribution


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Expression

BKB3 was detected in testis, pancreas and spleen (Xia [1182]).


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Functional

There are four splice variants (BKB3a-d), whose differences are in the NH2-terminal region. Each splice variant confers different inactivation properties to the MaxiK channel. Whereas the BKB3a and BKB3c subunits confer similar inactivation properties, BKB3b induces a faster and incomplete inactivation process that becomes evi- dent only at large depolarizations (Xia [1182]). It is unclear whether or not the BKB3d-subunit interacts with the alpha-subunit since coexpression of alpha- and BKB3d-subunits does not produce changes in the Ca2+ activation curves or in the gating kinetics of the MaxiK channel. (Orio [540])

The BKB3b auxiliary subunit, when coexpressed with the Slo alpha subunit, results in a particularly rapid (1‬ms), but incomplete inactivation, mediated by the cytosolic NH2 terminus of the BKB3b subunit (Xia et al., 2000 [1182]). See Lingle [1186] for by a two-step blocking mechanism of this process.


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Kinetics


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Model


References

1185

Hu S. et al. Variants of the KCNMB3 regulatory subunit of maxi BK channels affect channel inactivation.
Physiol. Genomics, 2003 Nov 11 , 15 (191-8).

540

Ferreira G. et al. New disguises for an old channel: MaxiK channel beta-subunits.
News Physiol. Sci., 2002 Aug , 17 (156-61).


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Credits