Referenced in: none

Automatically associated channels: Slo1 , Slo2

Title: Expression, purification and functional reconstitution of slack sodium-activated potassium channels.

Authors: Yangyang Yan, Youshan Yang, Shumin Bian, Fred J Sigworth

Journal, date & volume: J. Membr. Biol., 2012 Nov , 245, 667-74

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/22729647

Abstract
The slack (slo2.2) gene codes for a potassium-channel α-subunit of the 6TM voltage-gated channel family. Expression of slack results in Na(+)-activated potassium channel activity in various cell types. We describe the purification and reconstitution of Slack protein and show that the Slack α-subunit alone is sufficient for potassium channel activity activated by sodium ions as assayed in planar bilayer membranes and in membrane vesicles.